23.-25.5.2014 se v německém Ploenu konal workshop Wild House Mouse Meeting, kterého se zúčastnili 3 členové NextGen týmu a prezentovali zde své vědecké výsledky:
Genomics features associated with regions of high and low introgression in the house mouse hybrid zone
On May 23-25, I attended the international workshop on the evolutionary biology of the house mouse (Mus musculus), held in the Max-Plank Institute of Evolutionary Biology (MPI) in Ploen, Germany. I used this opportunity to learn about the current state of the research of the wild house mouse model system, but also to meet with collaborators Robert Karn and Christina Laukaitis and discuss the progress of our joint project on gene family size expansion in the mouse genome. I also established many contacts in the house mouse research community, and discussed, with director of the MPI Dr. Diethart Tautz, the possibility of collaboration in the laboratory of the MPI in Ploen to genotype the house mouse samples from the hybrid zone for the variants in gene copy number. The MPI in Ploen will welcome, in the near future, the researchers in mouse research team from Studenec for a working visit and will provide them with the use of necessary equipment and laboratory space to obtain the data on the gene copy number variants in their samples.
Stuart J. E. Baird:
Genome wide multilocus analysis of a hybrid zone
Stuart J.E. Baird, Alexey Yanchukov, Milos Macholan, Pavel Munclinger, Jaroslav Pialek
We use a hybrid zone with well-developed genomic resources to highlight what may soon be possible for non-model systems. The European House Mouse hybrid zone (HMHZ) is well studied, and the mouse and human genomes were completed in parallel due to the pivotal role of laboratory mice in medical research. This means mouse genome functional annotation is dense and cheap methods exist to recovery SNP variation at the population genomic scale. We combine 1400 SNPs for 1400 mice near the HMHZ and 0.5 million SNPs for 250 mice at a wider geographic scale to assess variation in gene flow permeability of the HMHZ species barrier along the mouse genome. As genome sampling and computational resources are no longer bottlenecked the challenge becomes making sound (model based) inference about the evolutionary process. Comparison with analyses using traditional datasets shows that the existing modelling framework of multilocus hybrid zone population genetics predicts genome wide patterns extremely well, providing a logical starting point for extensions that take advantage of higher resolution data. Dense SNPs allow co-estimation of source taxon and phase of DNA strands along chromosome pairs ie reconstruction of recombinant haplotypes as interdigitated blocks labelled by source. We illustrate how applying Fisher’s model of genome admixture can then distinguish ancestral polymorphism from introgressive, and allow us to move away from simplistic genome-outlier analyses and towards a model based understanding of the dynamics of introgression across a species barrier.